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KMID : 0881720190340030235
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Journal of Food Hygiene and Safety
2019 Volume.34 No. 3 p.235 ~ p.241
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Development of Analytical Method for Kasugamycin in Agricultural Products using LC-MS/MS
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Lee Han-Sol
Do Jung-Ah
Park Ji-Su
Cho Sung-Min
Shin Hye-Sun
Jang Dong-Eun
Jung Yong-Hyun
Lee Kang-Bong
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Abstract
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An analytical method was developed for the determination of an antibiotic fungicide, kasugamycin, in agricultural products (hulled rice, potato, soybean, mandarin and green pepper) using liquid chromatographytandem mass spectrometry (LC-MS/MS). Samples were extracted with methanol adjusted to pH 13 using 1 N sodium hydroxide, and purified with a HLB (hydrophilic lipophilic balance) cartridge. Linearity of a matrix-matched calibration curve using seven concentration levels, from 0.001 to 0.1 mg/kg, was excellent with a correlation coefficient (R2 ) of more than 0.9998. The limits of detection (LOD) and quantification (LOQ) of instrument were 0.0005 and 0.001 μg/mL, respectively, and the LOQ of analytical method calculated as 0.01 mg/kg. The average recoveries at three spiking levels (LOQ, LOQ×10, LOQ×50, n=5) were in the range of 71.2~95.4% with relative standard deviation of less than 12.1%. The developed method was simple and all optimized results was satisfied with the criteria ranges requested in the Codex guidelines and Food Safety Evaluation Department guidelines. The present study could be served as a reference for the establishment of maximum residue limits (MRL) of kasugamycin and be used as basic data for safety management relative to kasugamycin residues in imported and domestic agricultural products.
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KEYWORD
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Kasugamycin, Antibiotic fungicide, Agricultural product, Analytical method, LC-MS/MS
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